동물분자생체공학연구소 주관 초청강연 55
연사 : 유정은 박사 ((주)에스엘테라퓨틱스)
제목 : Purification of functional human PSC-derived midbrain dopaminergic precursors using cell surface markers
일시 : 2024년 4월 12일 금요일 오후 3시
장소 : 생명과학관 서관 201호
초록
Successful cell therapy for Parkinson’s disease (PD) requires large numbers of homogeneous ventral mesencephalic dopaminergic (vmDA) precursors. Enrichment of vmDA precursors via cell sorting is required to ensure high safety and efficacy of the cell therapy. Using LMX1A-eGFP knock-in reporter human embryonic stem cells, a novel surface antigen, trophoblast glycoprotein (TPBG), which was preferentially expressed in vmDA precursors was discovered. TPBG-targeted cell sorting enriched FOXA2+LMX1A+ vmDA precursors and helped attain efficient behavioral recovery of rodent PD models with increased numbers of TH+, NURR1+, and PITX3+ vmDA neurons in the grafts.